Detection of RAPD Markers Correlated with Chloroquine Resistance in Plasmodium falciparum | Zendy

Pierre Lescuyer | Zendy; Stéphane Picot | Zendy; Valérie Bracchi | Zendy; Josette Burnod | Zendy; Juliet Austin | Zendy; Alain Pérard | Zendy; P Ambroise-Thomas | Zendy

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Detection of RAPD Markers Correlated with Chloroquine Resistance in Plasmodium falciparum

Author(s) -

Pierre Lescuyer,

Stéphane Picot,

Valérie Bracchi,

Josette Burnod,

Juliet Austin,

Alain Pérard,

P Ambroise-Thomas

Publication year - 1997

Publication title -

genome research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.556

H-Index - 297

eISSN - 1549-5469

pISSN - 1088-9051

DOI - 10.1101/gr.7.7.747

Subject(s) - rapd , biology , primer (cosmetics) , plasmodium falciparum , genetics , genetic marker , polymerase chain reaction , dna , microbiology and biotechnology , gene , malaria , genetic diversity , population , immunology , demography , sociology , chemistry , organic chemistry

We described the use of the random amplified polymorphic DNA (RAPD) technique on Plasmodium falciparum DNA to detect genetic markers for chloroquine-resistant strains. Fourteen RAPD primers were tested, three of which generated banding patterns correlated with chloroquine resistance. To measure this correlation, the RAPD profiles were analyzed using the Nei and Li similarity coefficient. Detection of distinctive RAPD bands allowed us to synthesize specific PCR primers to be used on whole-blood samples. Two primer sets were synthesized and tested on sensitive and resistant strains for their ability to amplify the DNA fragment corresponding to the RAPD marker. These results suggest that RAPD and PCR techniques can be used as powerful tools for the detection of genetic markers associated with drug resistance.

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