Centromeric and Noncentromeric ADE2-Selectable Fragmentation Vectors for Yeast Artificial Chromosomes in AB1380 | Zendy

Joris J. Heus | Zendy; Menno P.J. de Winther | Zendy; Esther van de Vosse | Zendy; GertJan B. van Ommen | Zendy; Johan T. den Dunnen | Zendy

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Centromeric and Noncentromeric ADE2-Selectable Fragmentation Vectors for Yeast Artificial Chromosomes in AB1380

Author(s) -

Joris J. Heus,

Menno P.J. de Winther,

Esther van de Vosse,

GertJan B. van Ommen,

Johan T. den Dunnen

Publication year - 1997

Publication title -

genome research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.556

H-Index - 297

eISSN - 1549-5469

pISSN - 1088-9051

DOI - 10.1101/gr.7.6.657

Subject(s) - selectable marker , biology , genetics , yeast artificial chromosome , fragmentation (computing) , vector (molecular biology) , dna fragmentation , computational biology , dna , chromosome , plasmid , gene , gene mapping , recombinant dna , ecology , apoptosis , programmed cell death

We have constructed a set of fragmentation vectors for the truncation of either the centromeric or the noncentromeric end of YACs containing a human DNA insert. These vectors carry ADE2 or HIS5 as the selectable marker, enabling direct use in AB1380, the host strain of most publicly available YAC libraries. Centromeric fragmentation vectors for AB1380 have not been reported previously; the noncentromeric vectors show high frequencies of fragmentation.

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