A chromosome-specific microdissected library increases marker density on bovine chromosome 1. | Zendy

Tad S. Sonstegard | Zendy; F. Abel Ponce de León | Zendy; Craig W. Beattie | Zendy; S M Kappes | Zendy

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A chromosome-specific microdissected library increases marker density on bovine chromosome 1.

Author(s) -

Tad S. Sonstegard,

F. Abel Ponce de León,

Craig W. Beattie,

S M Kappes

Publication year - 1997

Publication title -

genome research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.556

H-Index - 297

eISSN - 1549-5469

pISSN - 1088-9051

DOI - 10.1101/gr.7.1.76

Subject(s) - biology , microsatellite , genetic marker , genetics , gene mapping , genetic linkage , chromosomal translocation , chromosome , quantitative trait locus , population , microbiology and biotechnology , linkage (software) , gene , allele , demography , sociology

Genetic resolution of bovine chromosome 1 (BTA1) linkage group was significantly increased by screening for microsatellite clones a microdissected library constructed from a bovine cell line carrying a t(1;29) translocation. Eighty-five percent of the microsatellites (ms) (46/54) identified were informative in the USDA/MARC mapping population, and 96% of these ms (44/46) linked to BTA1 (LOD > 3.0). When merged with 40 existing BTA1 markers the genetic map spanned 153.8 cM (sex-averaged interval, 1.9 cM). The fourfold improvement in marker density of BTA1 provides a genetic map that enhances mapping of quantitative trait loci and implementation of marker assisted selection.

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