Examining protein–protein interactions using endogenously tagged yeast arrays: The Cross-and-Capture system | Zendy

Bernhard Suter | Zendy; Michael J. Fetchko | Zendy; Ralph Imhof | Zendy; Christopher I. Graham | Zendy; Ingrid Stoffel-Studer | Zendy; Caroline Zbinden | Zendy; Maanasa Raghavan | Zendy; Lianet Lopez | Zendy; Lucija Beneti | Zendy; Jacqueline Hort | Zendy; Jeffrey Fillingham | Zendy; Jack Greenblatt | Zendy; Guri Giaever | Zendy; Corey Nislow | Zendy; Igor Štagljar | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Examining protein–protein interactions using endogenously tagged yeast arrays: The Cross-and-Capture system

Author(s) -

Bernhard Suter,

Michael J. Fetchko,

Ralph Imhof,

Christopher I. Graham,

Ingrid Stoffel-Studer,

Caroline Zbinden,

Maanasa Raghavan,

Lianet Lopez,

Lucija Beneti,

Jacqueline Hort,

Jeffrey Fillingham,

Jack Greenblatt,

Guri Giaever,

Corey Nislow,

Igor Štagljar

Publication year - 2007

Publication title -

genome research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.556

H-Index - 297

eISSN - 1549-5469

pISSN - 1088-9051

DOI - 10.1101/gr.6667007

Subject(s) - biology , yeast , computational biology , protein–protein interaction , protein fragment complementation assay , saccharomyces cerevisiae , function (biology) , genetics , dna , two hybrid screening , protein function , gene , phenotype , complementation

Comprehensive approaches to detect protein-protein interactions (PPIs) have been most successful in the yeast model system. Here we present "Cross-and-Capture," a novel assay for rapid, sensitive assessment of PPIs via pulldown of differently tagged yeast strain arrays. About 500 yeast genes that function in DNA replication, repair, and recombination and nuclear proteins of unknown function were chromosomally tagged with six histidine residues or triple VSV epitopes. We demonstrate that the assay can interrogate a wide range of previously known protein complexes with increased resolution and sensitivity. Furthermore, we use "Cross-and-Capture" to identify two novel protein complexes: Rtt101p-Mms1p and Sae2p-Mre11p. The Rtt101p-Mms1p interaction was subsequently characterized by genetic and functional analyses. Our studies establish the "Cross-and-Capture" assay as a novel, versatile tool that provides a valuable complement for the next generation of yeast proteomic studies.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research