Uniform amplification of a mixture of deoxyribonucleic acids with varying GC content. | Zendy

Namadev Baskaran | Zendy; Rajendra P. Kandpal | Zendy; A.K. Bhargava | Zendy; Michael W. Glynn | Zendy; A. Bale | Zendy; S M Weissman | Zendy

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Uniform amplification of a mixture of deoxyribonucleic acids with varying GC content.

Author(s) -

Namadev Baskaran,

Rajendra P. Kandpal,

A.K. Bhargava,

Michael W. Glynn,

A. Bale,

S M Weissman

Publication year - 1996

Publication title -

genome research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.556

H-Index - 297

eISSN - 1549-5469

pISSN - 1088-9051

DOI - 10.1101/gr.6.7.633

Subject(s) - biology , gc content , multiple displacement amplification , dna , polymerase chain reaction , betaine , microbiology and biotechnology , polymerase , genetics , biochemistry , dna extraction , gene , genome

A PCR method for uniform amplification of a mixture of DNA templates differing in GC content is described using the two enzyme approach (Klentaq1 and Pfu DNA polymerase) and a combination of DMSO and betaine. This method was applied to amplify the CGG repeat region from the fragile X region.

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