Construction of a YAC contig encompassing the Usher syndrome type 1C and familial hyperinsulinism loci on chromosome 11p14-15.1. | Zendy

Radha Ayyagari | Zendy; Ann Nestorowicz | Zendy; Yunjian Li | Zendy; Settara C. Chandrasekharappa | Zendy; Craig Chinault | Zendy; Peter van Tuinen | Zendy; Richard J. Smith | Zendy; J. Fielding Hejtmancik | Zendy; M. Alan Permutt | Zendy

Open Access

Construction of a YAC contig encompassing the Usher syndrome type 1C and familial hyperinsulinism loci on chromosome 11p14-15.1.

Author(s) -

Radha Ayyagari,

Ann Nestorowicz,

Yunjian Li,

Settara C. Chandrasekharappa,

Craig Chinault,

Peter van Tuinen,

Richard J. Smith,

J. Fielding Hejtmancik,

M. Alan Permutt

Publication year - 1996

Publication title -

genome research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.556

H-Index - 297

eISSN - 1549-5469

pISSN - 1088-9051

DOI - 10.1101/gr.6.6.504

Subject(s) - contig , biology , yeast artificial chromosome , genetics , chromosome , gene mapping , sequence tagged site , usher syndrome , gene , genome , retinitis pigmentosa

The Usher syndrome type 1C (USH1C) and familial hyperinsulinism (HI) loci have been assigned to chromosome 11p14-15.1, within the interval D11S419-D11S1310. We have constructed a yeast artificial chromosome (YAC) contig, extending from D11S926 to D11S899, which encompasses the critical regions for both USH1C and HI and spans an estimated genetic distance of approximately 4 cM. A minimal set of six YAC clones constitute the contig, with another 22 YACs confirming the order of sequence-tagged sites (STSs) and position of YACs on the contig. A total of 40 STSs, including 10 new STSs generated from YAC insert-end sequences and inter-Alu PCR products, were used to order the clones within the contig. This physical map provides a resource for identification of gene transcripts associated with USH1C, HI, and other genetic disorders that map to the D11S926-D11S899 interval.

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