Discovering distinct genes represented in 29,570 clones from infant brain cDNA libraries by applying sequencing by hybridization methodology. | Zendy

Aleksandar Milosavljević | Zendy; Marija Zeremski | Zendy; Žaklina Strezoska | Zendy; Danica Grujić | Zendy; H Dyanov | Zendy; Shawna Batus | Zendy; David Salbego | Zendy; Tatjana Paunesku | Zendy; Marcelo B. Soares | Zendy; Radomir Crkvenjakov | Zendy

Open Access

Discovering distinct genes represented in 29,570 clones from infant brain cDNA libraries by applying sequencing by hybridization methodology.

Author(s) -

Aleksandar Milosavljević,

Marija Zeremski,

Žaklina Strezoska,

Danica Grujić,

H Dyanov,

Shawna Batus,

David Salbego,

Tatjana Paunesku,

Marcelo B. Soares,

Radomir Crkvenjakov

Publication year - 1996

Publication title -

genome research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.556

H-Index - 297

eISSN - 1549-5469

pISSN - 1088-9051

DOI - 10.1101/gr.6.2.132

Subject(s) - biology , genbank , complementary dna , cdna library , genetics , gene , expressed sequence tag , computational biology , oligonucleotide , dna sequencing , sequence (biology) , oligomer restriction

To discover all distinct human genes and to determine their patterns of expression across different cell types, developmental stages, and physiological conditions, a procedure is needed for fast, mutual comparison of hundreds of thousands (and perhaps millions) of clones from cDNA libraries, as well as their comparison against data bases of sequenced DNA. In a pilot study, 29,570 clones in duplicate from both original and normalized, directional, infant brain cDNA libraries were hybridized with 107-215 heptamer oligonucleotide probes to obtain oligonucleotide sequence signatures (OSSs). The OSSs were compared and clustered based on mutual similarity into 16,741 clusters, each corresponding to a distinct cDNA. A number of distinct cDNAs were successfully recognized by matching their 107-probe OSSs against GenBank entries, indicating the possibility of sequence recognition with only a few hundred randomly chosen oligomers.

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