An ordered yeast artificial chromosome library covering over half of rice chromosome 6. | Zendy

Yosuke Umehara | Zendy; Hiroshi Tanoue | Zendy; N. Kurata | Zendy; Ikuo Ashikawa | Zendy; Y. Minobe | Zendy; Takashi Sasaki | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

An ordered yeast artificial chromosome library covering over half of rice chromosome 6.

Author(s) -

Yosuke Umehara,

Hiroshi Tanoue,

N. Kurata,

Ikuo Ashikawa,

Y. Minobe,

Takashi Sasaki

Publication year - 1996

Publication title -

genome research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.556

H-Index - 297

eISSN - 1549-5469

pISSN - 1088-9051

DOI - 10.1101/gr.6.10.935

Subject(s) - biology , contig , yeast artificial chromosome , genetics , gene mapping , sequence tagged site , chromosome , genome , genetic marker , genomic library , restriction fragment length polymorphism , genetic linkage , positional cloning , chromosome 22 , chromosome 4 , bacterial artificial chromosome , gene , locus (genetics) , polymerase chain reaction , base sequence

Yeast artificial chromosome (YAC) clones carrying DNA marker sequences located on the rice genetic map of chromosome 6 were ordered for physical mapping. A total of 122 restriction fragment length polymorphism markers, 16 sequence-tagged site markers, and five random amplified polymorphic DNA markers located, on average, at 0.9-cM intervals, were used for YAC clone screening by colony/Southern hybridization and PCR screening, respectively. A total of 216 individual YACs were selected from our YAC library of 7000 clones covering six genome equivalents. Each DNA marker could select, on average, 4.8 YAC clones, with 11 clones being the maximum. The YACs localized to the corresponding linkage map positions form 43 contigs and encompass about 60% of rice chromosome 6. This is the first step in constructing a physical map covering the whole rice genome by chromosome landing with YAC clones. These YACs and data will be used soon to isolate phenotypical trait genes by map-based cloning.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research