RNA-primed PCR. | Zendy

Hiroki Shibata | Zendy; Tomoko Tahira | Zendy; Kenshi Hayashi | Zendy

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RNA-primed PCR.

Author(s) -

Hiroki Shibata,

Tomoko Tahira,

Kenshi Hayashi

Publication year - 1995

Publication title -

genome research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.556

H-Index - 297

eISSN - 1549-5469

pISSN - 1088-9051

DOI - 10.1101/gr.5.4.400

Subject(s) - biology , primer (cosmetics) , reverse transcriptase , rna , microbiology and biotechnology , dna , polymerase , transcription (linguistics) , rna directed dna polymerase , rna dependent rna polymerase , nucleotide , applications of pcr , genetics , polymerase chain reaction , gene , digital polymerase chain reaction , linguistics , chemistry , philosophy , organic chemistry

We show that RNA can serve as a primer in PCR. Use of rTth DNA polymerase is essential because it has strong reverse transcriptase activity. RNA primers can be obtained by in vitro transcription and are less costly than DNA primers, which are chemically synthesized. RNA-primed PCR also opens the possibility that a specific amplification reaction can be achieved in the absence of knowledge of the target nucleotide sequence.

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