Screening of Gene-Associated Polymorphisms by Use of In-Gel Competitive Reassociation and EST (cDNA) Array Hybridization | Zendy

Koshichi Gotoh | Zendy; Michio Oishi | Zendy

Open Access

Screening of Gene-Associated Polymorphisms by Use of In-Gel Competitive Reassociation and EST (cDNA) Array Hybridization

Author(s) -

Koshichi Gotoh,

Michio Oishi

Publication year - 2003

Publication title -

genome research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.556

H-Index - 297

eISSN - 1549-5469

pISSN - 1088-9051

DOI - 10.1101/gr.434103

Subject(s) - biology , genetics , complementary dna , gene , dna , dna–dna hybridization , restriction fragment length polymorphism , nucleic acid thermodynamics , microbiology and biotechnology , dna sequencing , computational biology , polymerase chain reaction , base sequence

In-gel competitive reassociation (IGCR) is a method of differential subtraction to enrich polymorphic DNA restriction fragments between two DNA samples without probes or specific sequence information. Here, we show that by combining IGCR and expressed sequence tags (EST) array hybridization, polymorphic DNA fragments associated with genes in complex higher organisms (Arabidopsis thaliana) can be effectively screened, demonstrating that this procedure offers a simple and efficient way to obtain gene-associated polymorphic DNA markers.

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