Simple, robust methods for high-throughput nanoliter-scale DNA sequencing | Zendy

Duane E. Smailus | Zendy; Andre Marziali | Zendy; Philip Dextras | Zendy; Marco A. Marra | Zendy; Robert A. Holt | Zendy

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Simple, robust methods for high-throughput nanoliter-scale DNA sequencing

Author(s) -

Duane E. Smailus,

Andre Marziali,

Philip Dextras,

Marco A. Marra,

Robert A. Holt

Publication year - 2005

Publication title -

genome research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.556

H-Index - 297

eISSN - 1549-5469

pISSN - 1088-9051

DOI - 10.1101/gr.4221805

Subject(s) - biology , fosmid , throughput , dna sequencing , computational biology , dna , genome , genetics , computer science , gene , operating system , wireless

We have developed high-throughput DNA sequencing methods that generate high quality data from reactions as small as 400 nL, providing an approximate order of magnitude reduction in reagent use relative to standard protocols. Sequencing of clones from plasmid, fosmid, and BAC libraries yielded read lengths (PHRED20 bases) of 765 +/- 172 (n = 10,272), 621 +/- 201 (n = 1824), and 647 +/- 189 (n = 568), respectively. Implementation of these procedures at high-throughput genome centers could have a substantial impact on the amount of data that can be generated per unit cost.

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