Efficient gene synthesis by Klenow assembly/extension-Pfu polymerase amplification (KAPPA) of overlapping oligonucleotides.
Author(s) -
Eugene W. Holowachuk,
Mary S. Ruhoff
Publication year - 1995
Publication title -
genome research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.556
H-Index - 297
eISSN - 1549-5469
pISSN - 1088-9051
DOI - 10.1101/gr.4.5.299
Subject(s) - biology , klenow fragment , oligonucleotide , microbiology and biotechnology , applications of pcr , polymerase chain reaction , dna polymerase i , genetics , polymerase , gene , computational biology , reverse transcriptase , exonuclease , multiplex polymerase chain reaction
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