Open AccessA Scalable High-Throughput Chemical Synthesizer
Author(s) -
Eric A. Livesay,
Ying Horng Liu,
Kevin J Luebke,
Joel Irick,
Yuri Belosludtsev,
Simon Rayner,
Robert P Balog,
Stephen Albert Johnston
Publication year - 2002
Publication title -
genome research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.556
H-Index - 297
eISSN - 1549-5469
pISSN - 1088-9051
DOI - 10.1101/gr.359002
Subject(s) - scalability , throughput , biomolecule , computer science , biology , reagent , process (computing) , computational biology , nanotechnology , materials science , chemistry , telecommunications , database , wireless , operating system
A machine that employs a novel reagent delivery technique for biomolecular synthesis has been developed. This machine separates the addressing of individual synthesis sites from the actual process of reagent delivery by using masks placed over the sites. Because of this separation, this machine is both cost-effective and scalable, and thus the time required to synthesize 384 or 1536 unique biomolecules is very nearly the same. Importantly, the mask design allows scaling of the number of synthesis sites without the addition of new valving. Physical and biological comparisons between DNA made on a commercially available synthesizer and this unit show that it produces DNA of similar quality.