Genotyping ocular strains of Chlamydia trachomatis by single-tube nested PCR. | Zendy

S Pecharatana | Zendy; Mark A. Pickett | Zendy; P. J. Watt | Zendy; Michael E. Ward | Zendy

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Genotyping ocular strains of Chlamydia trachomatis by single-tube nested PCR.

Author(s) -

S Pecharatana,

Mark A. Pickett,

P. J. Watt,

Michael E. Ward

Publication year - 1993

Publication title -

genome research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.556

H-Index - 297

eISSN - 1549-5469

pISSN - 1088-9051

DOI - 10.1101/gr.3.3.200

Subject(s) - biology , genotyping , chlamydia trachomatis , serotype , nested polymerase chain reaction , genotype , polymerase chain reaction , virology , primer (cosmetics) , genetics , gene , chemistry , organic chemistry

A single-tube nested PCR able to discriminate between ocular infection with Chlamydia trachomatis of serovar A, B, or C is described. The method uses genotype-specific primers labeled with different fluorochromes, "drop-in/drop-out" PCR amplification, and product analysis on an Applied Biosystems 373A DNA Sequencer using GENESCAN 672 software. The system readily detected mixed infection with serotypes A and B within the same eye. This strategy provides a rapid genotyping method applicable to a wide variety of epidemiological studies.

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