Quantitative aspects of the mutant analysis by PCR and restriction enzyme cleavage (MAPREC). | Zendy

Zhengbin Lu | Zendy; Monica Douthitt | Zendy; Rolf E. Taffs | Zendy; Yuxin Ran | Zendy; Laurie P. Norwood | Zendy; Konstantin Chumakov | Zendy

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Quantitative aspects of the mutant analysis by PCR and restriction enzyme cleavage (MAPREC).

Author(s) -

Zhengbin Lu,

Monica Douthitt,

Rolf E. Taffs,

Yuxin Ran,

Laurie P. Norwood,

Konstantin Chumakov

Publication year - 1993

Publication title -

genome research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.556

H-Index - 297

eISSN - 1549-5469

pISSN - 1088-9051

DOI - 10.1101/gr.3.3.176

Subject(s) - biology , heteroduplex , cleavage (geology) , restriction enzyme , mutant , genetics , polymerase chain reaction , microbiology and biotechnology , virology , dna , gene , paleontology , fracture (geology)

Quantitation of virus revertants by PCR and restriction enzyme cleavage may give nonlinear results and, in some cases, produce artifacts caused by nucleotide misincorporation and heteroduplex formation, occurring during PCR. Modifications of the procedure allowed us to overcome these problems and develop a highly sensitive and reliable method of mutant quantitation. This procedure can be used to assess the quality of live vaccines and to study heterogeneity of viral and bacterial populations.

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