A method for accurate amplification of polymorphic CA-repeat sequences. | Zendy

Shan J. Odelberg | Zendy; Richard White | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

A method for accurate amplification of polymorphic CA-repeat sequences.

Author(s) -

Shan J. Odelberg,

Richard White

Publication year - 1993

Publication title -

genome research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.556

H-Index - 297

eISSN - 1549-5469

pISSN - 1088-9051

DOI - 10.1101/gr.3.1.7

Subject(s) - biology , yield (engineering) , polyacrylamide gel electrophoresis , genetics , gel electrophoresis , polymerase chain reaction , microbiology and biotechnology , computational biology , gene , biochemistry , materials science , metallurgy , enzyme

Anomalous PCR products are often produced during the amplification of d(CA)n.d(TG)n sequences. Upon denaturing polyacrylamide gel electrophoresis, these products yield a ladder-like pattern that can complicate genotypic interpretation. We have developed two related techniques, referred to as two- and three-stage linear amplification (2-SLA and 3-SLA, respectively), which largely overcome this problem and yield readily interpretable banding patterns.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research