An Efficient SNP System for Mouse Genome Scanning and Elucidating Strain Relationships | Zendy

Petko M. Petkov | Zendy; Yueming Ding | Zendy; Megan A. Cassell | Zendy; Weidong Zhang | Zendy; Gunjan Wagner | Zendy; Evelyn E. Sargent | Zendy; Steven Asquith | Zendy; Victor Crew | Zendy; Kevin A. Johnson | Zendy; Phil Robinson | Zendy; Valerie E. Scott | Zendy; Michael V. Wiles | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

An Efficient SNP System for Mouse Genome Scanning and Elucidating Strain Relationships

Author(s) -

Petko M. Petkov,

Yueming Ding,

Megan A. Cassell,

Weidong Zhang,

Gunjan Wagner,

Evelyn E. Sargent,

Steven Asquith,

Victor Crew,

Kevin A. Johnson,

Phil Robinson,

Valerie E. Scott,

Michael V. Wiles

Publication year - 2004

Publication title -

genome research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.556

H-Index - 297

eISSN - 1549-5469

pISSN - 1088-9051

DOI - 10.1101/gr.2825804

Subject(s) - biology , genetics , genotyping , inbred strain , strain (injury) , genome , subspecies , quantitative trait locus , snp , laboratory mouse , allele , genetic marker , genotype , gene , single nucleotide polymorphism , paleontology , anatomy

A set of 1638 informative SNP markers easily assayed by the Amplifluor genotyping system were tested in 102 mouse strains, including the majority of the common and wild-derived inbred strains available from The Jackson Laboratory. Selected from publicly available databases, the markers are on average approximately 1.5 Mb apart and, whenever possible, represent the rare allele in at least two strains. Amplifluor assays were developed for each marker and performed on two independent DNA samples from each strain. The mean number of polymorphisms between strains was 608+/-136 SD. Several tests indicate that the markers provide an effective system for performing genome scans and quantitative trait loci analyses in all but the most closely related strains. Additionally, the markers revealed several subtle differences between closely related mouse strains, including the groups of several 129, BALB, C3H, C57, and DBA strains, and a group of wild-derived inbred strains representing several Mus musculus subspecies. Applying a neighbor-joining method to the data, we constructed a mouse strain family tree, which in most cases confirmed existing genealogies.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research