A Gene Expression Screen in Zebrafish Embryogenesis | Zendy

Tetsuhiro Kudoh | Zendy; Michael Tsang | Zendy; Neil A. Hukriede | Zendy; Xiongfong Chen | Zendy; Michael Dedekian | Zendy; Christopher J. Clarke | Zendy; Anne Kiang | Zendy; Stephanie A. Schultz | Zendy; Jonathan A. Epstein | Zendy; Reiko Toyama | Zendy; Igor B. Dawid | Zendy

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A Gene Expression Screen in Zebrafish Embryogenesis

Author(s) -

Tetsuhiro Kudoh,

Michael Tsang,

Neil A. Hukriede,

Xiongfong Chen,

Michael Dedekian,

Christopher J. Clarke,

Anne Kiang,

Stephanie A. Schultz,

Jonathan A. Epstein,

Reiko Toyama,

Igor B. Dawid

Publication year - 2001

Publication title -

genome research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.556

H-Index - 297

eISSN - 1549-5469

pISSN - 1088-9051

DOI - 10.1101/gr.209601

Subject(s) - biology , zebrafish , somitogenesis , genetics , in situ hybridization , gene , cdna library , embryogenesis , embryo , complementary dna , expressed sequence tag , gastrulation , computational biology , gene expression , microbiology and biotechnology , somite

A screen for developmentally regulated genes was conducted in the zebrafish, a system offering substantial advantages for the study of the molecular genetics of vertebrate embryogenesis. Clones from a normalized cDNA library from early somitogenesis stages were picked randomly and tested by high-throughput in situ hybridization for restricted expression in at least one of four stages of development. Among 2765 clones that were screened, a total of 347 genes with patterns judged to be restricted were selected. These clones were subjected to partial sequence analysis, allowing recognition of functional motifs in 163 among them. In addition, a portion of the clones were mapped with the aid of the LN54 radiation hybrid panel. The usefulness of the in situ hybridization screening approach is illustrated by describing several new markers for the characteristic structure in the fish embryo named the yolk syncytial layer, and for different regions of the developing brain.

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