Integration of the Rat Recombination and EST Maps in the Rat Genomic Sequence and Comparative Mapping Analysis With the Mouse Genome | Zendy

Steven P. Wilder | Zendy; MarieThérèse Bihoreau | Zendy; Karène Argoud | Zendy; Takeshi Watanabe | Zendy; Mark Lathrop | Zendy; Dominique Gauguier | Zendy

Open Access

Integration of the Rat Recombination and EST Maps in the Rat Genomic Sequence and Comparative Mapping Analysis With the Mouse Genome

Author(s) -

Steven P. Wilder,

MarieThérèse Bihoreau,

Karène Argoud,

Takeshi Watanabe,

Mark Lathrop,

Dominique Gauguier

Publication year - 2004

Publication title -

genome research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.556

H-Index - 297

eISSN - 1549-5469

pISSN - 1088-9051

DOI - 10.1101/gr.2001604

Subject(s) - biology , quantitative trait locus , genetics , expressed sequence tag , genome , microsatellite , genetic linkage , locus (genetics) , whole genome sequencing , gene mapping , inbred strain , computational biology , gene , allele , chromosome

Inbred strains of the laboratory rat are widely used for identifying genetic regions involved in the control of complex quantitative phenotypes of biomedical importance. The draft genomic sequence of the rat now provides essential information for annotating rat quantitative trait locus (QTL) maps. Following the survey of unique rat microsatellite (11,585 including 1648 new markers) and EST (10,067) markers currently available, we have incorporated a selection of 7952 rat EST sequences in an improved version of the integrated linkage-radiation hybrid map of the rat containing 2058 microsatellite markers which provided over 10,000 potential anchor points between rat QTL and the genomic sequence of the rat. A total of 996 genetic positions were resolved (avg. spacing 1.77 cM) in a single large intercross and anchored in the rat genomic sequence (avg. spacing 1.62 Mb). Comparative genome maps between rat and mouse were constructed by successful computational alignment of 6108 mapped rat ESTs in the mouse genome. The integration of rat linkage maps in the draft genomic sequence of the rat and that of other species represents an essential step for translating rat QTL intervals into human chromosomal targets.

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