Genome walking with 2- to 4-kb steps using panhandle PCR. | Zendy

Douglas H. Jones | Zendy; Stanley C. Winistorfer | Zendy

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Genome walking with 2- to 4-kb steps using panhandle PCR.

Author(s) -

Douglas H. Jones,

Stanley C. Winistorfer

Publication year - 1993

Publication title -

genome research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.556

H-Index - 297

eISSN - 1549-5469

pISSN - 1088-9051

DOI - 10.1101/gr.2.3.197

Subject(s) - biology , dna , genomic dna , primer (cosmetics) , human genome , genetics , computational biology , genome , primer dimer , dna sequencing , polymerase chain reaction , sequence (biology) , gene , multiplex polymerase chain reaction , chemistry , organic chemistry

Panhandle PCR is a method that permits the rapid retrieval of DNA segments flanking a known DNA sequence. This method extends the application of PCR to the retrieval of DNA where only one end of the DNA sequence is known, so that one can use PCR to walk along an uncharacterized stretch of DNA. In this report, we demonstrate that this method can be applied to the highly specific retrieval of large pieces (2.2-4.4 kb) of human genomic DNA that flank the primer annealing sites.

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