A lock-docking oligo(dT) primer for 5' and 3' RACE PCR. | Zendy

Nancy D. Borson | Zendy; W.L. Salo | Zendy; Lester R. Drewes | Zendy

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A lock-docking oligo(dT) primer for 5' and 3' RACE PCR.

Author(s) -

Nancy D. Borson,

W.L. Salo,

Lester R. Drewes

Publication year - 1992

Publication title -

genome research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.556

H-Index - 297

eISSN - 1549-5469

pISSN - 1088-9051

DOI - 10.1101/gr.2.2.144

Subject(s) - biology , primer (cosmetics) , complementary dna , rapid amplification of cdna ends , genetics , computational biology , gene , dock , sequence (biology) , microbiology and biotechnology , polymerase chain reaction , molecular cloning , chemistry , organic chemistry

We describe a method that can be used to obtain and sequence 3' and 5' ends of cDNA transcripts directly from PCR products. The method employs a modified oligo(dT) primer that enables it to "lock-dock" at the junction of gene-specific cDNA sequence and a natural (3') or appended (5') poly(A) tail. As a result, discrete, first-round PCR products are obtained that are easily isolated and sequenced directly.

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