Identification of Genes Encoding Amino Acid Permeases by Inactivation of Selected ORFs from the Synechocystis Genomic Sequence | Zendy

Maria Quintero | Zendy; Marı́a Luz Montesinos | Zendy; Antonia Herrero | Zendy; Enrique Flores | Zendy

Open Access

Identification of Genes Encoding Amino Acid Permeases by Inactivation of Selected ORFs from the Synechocystis Genomic Sequence

Author(s) -

Maria Quintero,

Marı́a Luz Montesinos,

Antonia Herrero,

Enrique Flores

Publication year - 2001

Publication title -

genome research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.556

H-Index - 297

eISSN - 1549-5469

pISSN - 1088-9051

DOI - 10.1101/gr.196301

Subject(s) - permease , orfs , biology , synechocystis , biochemistry , amino acid , atp binding cassette transporter , gene , peptide sequence , histidine , open reading frame , genetics , transporter , mutant

Genes encoding elements of four amino acid permeases were identified by insertional inactivation of ORFs from the genomic sequence of the cyanobacterium Synechocystis sp. strain PCC 6803 whose putative products are homologous to amino acid permease proteins from other bacteria. A transport system for neutral amino acids and histidine and a transport system for basic amino acids and glutamine were identified as ABC-type transporters, whereas Na(+)-dependent transport of glutamate was found to be mediated by at least two systems, the secondary permease GltS and a TRAP-type transporter. Except for GltS, substrate specificities of the identified permeases do not match those of previously characterized systems homologous to these permeases.

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