English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Tools annual

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Presents the access of premium content as premium feature

Premium Content

Global: Zendy Plus annual

Global: Zendy Free Plan

Pre-mRNA 5′ spliced-leader (SL)  trans -splicing occurs in some metazoan groups but not in others. Genome-wide characterization of the  trans -spliced mRNA subpopulation has not yet been reported for any metazoan. We carried out a high-throughput analysis of the SL  trans -spliced mRNA population of the ascidian tunicate  Ciona intestinalis  by 454 Life Sciences (Roche) pyrosequencing of SL-PCR-amplified random-primed reverse transcripts of tailbud embryo RNA. We obtained ∼250,000 high-quality reads corresponding to 8790 genes, ∼58% of the  Ciona  total gene number. The great depth of this data revealed new aspects of  trans -splicing, including the existence of a significant class of “infrequently  trans -spliced” genes, accounting for ∼28% of represented genes, that generate largely non- trans -spliced mRNAs, but also produce  trans -spliced mRNAs, in part through alternative promoter use. Thus, the conventional qualitative dichotomy of  trans -spliced versus non- trans -spliced genes should be supplanted by a more accurate quantitative view recognizing frequently and infrequently  trans -spliced gene categories. Our data include reads representing ∼80% of  Ciona  frequently  trans -spliced genes. Our analysis also revealed significant use of closely spaced alternative  trans -splice acceptor sites which further underscores the mechanistic similarity of  cis - and  trans -splicing and indicates that the prevalence of ±3-nt alternative splicing events at tandem acceptor sites, NAGNAG, is driven by spliceosomal mechanisms, and not nonsense-mediated decay, or selection at the protein level. The breadth of gene representation data enabled us to find new correlations between  trans -splicing status and gene function, namely the overrepresentation in the frequently  trans -spliced gene class of genes associated with plasma/endomembrane system, Ca 2+  homeostasis, and actin cytoskeleton.

genome research

High-throughput sequence analysis of <i>Ciona intestinalis</i> SL <i>trans</i>-spliced mRNAs: Alternative expression modes and gene function correlates

High-throughput sequence analysis of Ciona intestinalis SL trans-spliced mRNAs: Alternative expression modes and gene function correlates