Open AccessEffect of different laboratory techniques for guanidinium-phenol-chloroform RNA extraction on A260/A280 and on accuracy of mRNA quantitation by reverse transcriptase-PCR.
Author(s) -
Masahiko Yamaguchi,
Carl W. Dieffenbach,
Richard J. Connolly,
D F Cruess,
Wendy Baur,
John B. Sharefkin
Publication year - 1992
Publication title -
genome research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.556
H-Index - 297
eISSN - 1549-5469
pISSN - 1088-9051
DOI - 10.1101/gr.1.4.286
Subject(s) - biology , rna extraction , reverse transcriptase , messenger rna , rna , chloroform , computational biology , real time polymerase chain reaction , polymerase chain reaction , microbiology and biotechnology , extraction (chemistry) , reverse transcription polymerase chain reaction , phenol , chromatography , gene , genetics , chemistry , organic chemistry
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