Simplified template preparation and improved direct sequencing using Taq polymerase. | Zendy

Anand Srivastava | Zendy; V. Montanaro | Zendy; Juha Kere | Zendy

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Simplified template preparation and improved direct sequencing using Taq polymerase.

Author(s) -

Anand Srivastava,

V. Montanaro,

Juha Kere

Publication year - 1992

Publication title -

genome research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.556

H-Index - 297

eISSN - 1549-5469

pISSN - 1088-9051

DOI - 10.1101/gr.1.4.255

Subject(s) - biology , computational biology , taq polymerase , multiple displacement amplification , polymerase chain reaction , dna sequencing , applications of pcr , genetics , digital polymerase chain reaction , dna , thermus aquaticus , dna extraction , gene

A streamlined version of direct dideoxy sequencing is presented that includes template preparation as well as sequencing protocols. The method is used routinely to sequence double-stranded PCR products after minimal purification with one of the primers used in amplification. Either 35S or 32P labeling can be used with equally good results.

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