A sensitive, quantitative assay for measurement of allele-specific transcripts differing by a single nucleotide. | Zendy

Judith Singer–Sam | Zendy; Jeanne M. LeBon | Zendy; Aihua Dai | Zendy; Arthur D. Riggs | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

A sensitive, quantitative assay for measurement of allele-specific transcripts differing by a single nucleotide.

Author(s) -

Judith Singer–Sam,

Jeanne M. LeBon,

Aihua Dai,

Arthur D. Riggs

Publication year - 1992

Publication title -

genome research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.556

H-Index - 297

eISSN - 1549-5469

pISSN - 1088-9051

DOI - 10.1101/gr.1.3.160

Subject(s) - biology , primer extension , nucleotide , primer (cosmetics) , genetics , microbiology and biotechnology , allele , rna , single nucleotide polymorphism , gene , genotype , chemistry , organic chemistry

We have found that the single nucleotide primer extension assay, a PCR-based assay currently used qualitatively to measure allelic differences in DNA, can be used quantitatively to measure allele-specific transcripts differing by only a single nucleotide. We show that total RNA containing the Pgk-1a transcript can be specifically detected even in a 1000-fold excess of RNA containing the Pgk-1b transcript.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research