Open AccessA PCR-enhanced method for determining the 5' end sequence of mRNAs.
Author(s) -
Martin HofmannApitius,
David A. Brian
Publication year - 1991
Publication title -
genome research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.556
H-Index - 297
eISSN - 1549-5469
pISSN - 1088-9051
DOI - 10.1101/gr.1.1.43
Subject(s) - biology , sequence (biology) , complementary dna , messenger rna , cloning (programming) , computational biology , rna , microbiology and biotechnology , sequence analysis , genetics , gene , computer science , programming language
A method was developed to amplify ligated cDNA copies of mRNA 5' ends before cloning and sequencing. The method facilitates the study of the 5' end sequence on many molecules of an individual mRNA species. It can also be used to study the 5' end sequence on other kinds of single-stranded RNA molecules.
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