Large-scale gene trapping in C57BL/6N mouse embryonic stem cells | Zendy

Gwenn M. Hansen | Zendy; Diane C. Markesich | Zendy; Michael B. Burnett | Zendy; Qichao Zhu | Zendy; Karen Dionne | Zendy; Lizabeth J. Richter | Zendy; Richard H. Finnell | Zendy; Arthur Sands | Zendy; Brian Zambrowicz | Zendy; Alejandro Abuin | Zendy

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Large-scale gene trapping in C57BL/6N mouse embryonic stem cells

Author(s) -

Gwenn M. Hansen,

Diane C. Markesich,

Michael B. Burnett,

Qichao Zhu,

Karen Dionne,

Lizabeth J. Richter,

Richard H. Finnell,

Arthur Sands,

Brian Zambrowicz,

Alejandro Abuin

Publication year - 2008

Publication title -

genome research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.556

H-Index - 297

eISSN - 1549-5469

pISSN - 1088-9051

DOI - 10.1101/gr.078352.108

Subject(s) - biology , embryonic stem cell , trapping , genetics , scale (ratio) , gene , stem cell , microbiology and biotechnology , ecology , physics , quantum mechanics

We report the construction and analysis of a mouse gene trap mutant resource created in the C57BL/6N genetic background containing more than 350,000 sequence-tagged embryonic stem (ES) cell clones. We also demonstrate the ability of these ES cell clones to contribute to the germline and produce knockout mice. Each mutant clone is identified by a genomic sequence tag representing the exact insertion location, allowing accurate prediction of mutagenicity and enabling direct genotyping of mutant alleles. Mutations have been identified in more than 10,000 genes and show a bias toward the first intron. The trapped ES cell lines, which can be requested from the Texas A&M Institute for Genomic Medicine, are readily available to the scientific community.

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