Open AccessSequences in the pea rbcS-3A gene have homology to constitutive mammalian enhancers but function as negative regulatory elements.
Author(s) -
Cris Kuhlemeier,
Robert Fluhr,
P J Green,
NamHai Chua
Publication year - 1987
Publication title -
genes and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.136
H-Index - 438
eISSN - 1549-5477
pISSN - 0890-9369
DOI - 10.1101/gad.1.3.247
Subject(s) - enhancer , biology , gene , homology (biology) , regulatory sequence , genetics , enhancer rnas , transcription (linguistics) , microbiology and biotechnology , regulation of gene expression , transcription factor , linguistics , philosophy
The pea rbcS-3A gene, which codes for a key component of the photosynthetic machinery, requires light for its expression. Analysis of chimeric constructs in transgenic tobacco plants has shown that a 280-bp fragment from the 5' noncoding region can act as a light-inducible transcriptional enhancer. Further characterization of this enhancer identifies a 58-bp sequence containing two regulatory elements that can decrease transcription in the dark. One has a high degree of homology to the SV40 core enhancer, the other to the adenovirus 5 E1A enhancer and the constitutive part of the human interferon-beta gene enhancer. Deletion of the 58-bp sequence uncovers additional light-responsive elements (LREs) located further upstream and downstream.