Detection of Heteromers Formed by Cannabinoid CB1, Dopamine D2, and Adenosine A2AG-Protein-Coupled Receptors by Combining Bimolecular Fluorescence Complementation and Bioluminescence Energy Transfer | Zendy

Gemma Navarro | Zendy; Paulina Carriba | Zendy; Jorge Gandí | Zendy; Francisco Ciruela | Zendy; Vicent Casadó | Zendy; Antoni Cortés | Zendy; Josefa Mallol | Zendy; Enric I. Canela | Zendy; Carmen Lluís | Zendy; Rafael Franco | Zendy

Open Access

Detection of Heteromers Formed by Cannabinoid CB₁, Dopamine D₂, and Adenosine A_2AG-Protein-Coupled Receptors by Combining Bimolecular Fluorescence Complementation and Bioluminescence Energy Transfer

Author(s) -

Gemma Navarro,

Paulina Carriba,

Jorge Gandí,

Francisco Ciruela,

Vicent Casadó,

Antoni Cortés,

Josefa Mallol,

Enric I. Canela,

Carmen Lluís,

Rafael Franco

Publication year - 2008

Publication title -

the scientific world journal

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.453

H-Index - 93

eISSN - 2356-6140

pISSN - 1537-744X

DOI - 10.1100/tsw.2008.136

Subject(s) - chemistry , d 1 , g protein coupled receptor , receptor , bimolecular fluorescence complementation , adenosine , bioluminescence , adenosine receptor , biophysics , stereochemistry , biochemistry , biology , yeast , agonist

Functional interactions in signaling occur between dopamine D2 (D2R) and cannabinoid CB1 (CB1R) receptors, between CB1R and adenosine A2A (A2AR) receptors, and between D2R and A2AR. Furthermore, direct molecular interactions have been reported for the pairs CB1R-D2R, A2AR-D2R, and CB1R-A2AR. Here a combination of bimolecular fluorescence complementation and bioluminescence energy transfer techniques was used to identify the occurrence of D2R-CB1R-A2AR hetero-oligomers in living cells.

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