Molluscan Shell Matrix Characterization by Preparative SDS-PAGE | Zendy

Frédéric Marin | Zendy

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Molluscan Shell Matrix Characterization by Preparative SDS-PAGE

Author(s) -

Frédéric Marin

Publication year - 2003

Publication title -

the scientific world journal

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.453

H-Index - 93

eISSN - 2356-6140

pISSN - 1537-744X

DOI - 10.1100/tsw.2003.30

Subject(s) - polyclonal antibodies , matrix (chemical analysis) , fractionation , characterization (materials science) , shell (structure) , dissolution , glycoprotein , chromatography , chemistry , antibody , biochemistry , materials science , nanotechnology , biology , organic chemistry , immunology , composite material

The glycoproteinaceous constituents of molluscan shell matrices usually resist chromatographical fractionation. We describe a protocol that overcomes this difficulty and permits collection of a large amount of shell proteins for further in vitro characterization. After dissolution of the mineral phase, the glycoproteins are fractionated "blind" on a preparative electrophoresis. They are subsequently detected with a polyclonal antibody raised against the whole matrix.

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