Purification of Parietal and Chief Cells from the Gastric Mucosa
Author(s) -
John M. Graham
Publication year - 2002
Publication title -
the scientific world journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.453
H-Index - 93
eISSN - 2356-6140
pISSN - 1537-744X
DOI - 10.1100/tsw.2002.853
Subject(s) - parietal cell , gastric mucosa , enterochromaffin like cell , foveolar cell , elutriation , mucus , gastric chief cell , iodixanol , stomach , gastric acid , chemistry , medicine , biology , organic chemistry , radiology , ecology , contrast medium
Acid-secreting parietal cells from the gastric mucosa are widely studied as a model in studies on ion transport. A discontinuous gradient of iodixanol has been found to be superior to earlier protocols using Nycodenz and this method, which removes a significant amount of contaminating cells and mucus is a very useful prelude to further purification by elutriation.
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