Purification of Gastric Mucosal ECL Cells from a Crude Elutriation Fraction | Zendy

John M. Graham | Zendy

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Purification of Gastric Mucosal ECL Cells from a Crude Elutriation Fraction

Author(s) -

John M. Graham

Publication year - 2002

Publication title -

the scientific world journal

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.453

H-Index - 93

eISSN - 2356-6140

pISSN - 1537-744X

DOI - 10.1100/tsw.2002.852

Subject(s) - elutriation , enterochromaffin like cell , enteroendocrine cell , parietal cell , gastric mucosa , chemistry , chromatography , endocrine system , stomach , biochemistry , hormone , organic chemistry

Acid-secreting parietal cells from the gastric mucosa are widely studied as a model in studies on ion transport and the endocrine/paracrine ECL cells effectively control parietal cell function. Discontinuous gradients of iodixanol for the purification of ECL cells were subsequently simplified to the use of a density barrier. This technique is now commonly used following initial centrifugal elutriation.

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