Isolation of Rat and Human Hippocampal Neuron Fractions in a Discontinuous Density Gradient | Zendy

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Isolation of Rat and Human Hippocampal Neuron Fractions in a Discontinuous Density Gradient

Author(s) -

John Graham

Publication year - 2002

Publication title -

the scientific world journal

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.453

H-Index - 93

eISSN - 2356-6140

pISSN - 1537-744X

DOI - 10.1100/tsw.2002.850

Subject(s) - iodixanol , hippocampal formation , isolation (microbiology) , plating (geology) , neuron , brain tissue , density gradient , human brain , chemistry , anatomy , neuroscience , biology , medicine , bioinformatics , physics , paleontology , radiology , quantum mechanics , contrast medium

The plating efficiency of neurons in culture is highly dependent on the concentration of cells used to establish the monolayer. A discontinuous iodixanol gradient permits both the production of a viable concentrated suspension of neurons and purification from other brain tissue elements. The gradient that is described in this Protocol Article is applicable to brain tissue from rat and also from human biopsy specimens.

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