Homogenization of Mammalian Tissues
Author(s) -
John M. Graham
Publication year - 2002
Publication title -
the scientific world journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.453
H-Index - 93
eISSN - 2356-6140
pISSN - 1537-744X
DOI - 10.1100/tsw.2002.849
Subject(s) - homogenization (climate) , computer science , fractionation , soft tissue , liver tissue , chemistry , pathology , biology , chromatography , medicine , biodiversity , ecology , endocrinology
Satisfactory homogenization of a tissue is a necessary prerequisite to any fractionation schedule. A detailed protocol is given for rat liver because of the widespread use of this tissue. Although this technique should be broadly applicable to any soft tissue and to any subsequent fractionation procedure, there are certain tissues and applications that require either minor or extensive modification. Some of these points are addressed in the Notes section.
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