Open AccessIsolation of a Mouse Motoneuron-Enriched Fraction from Mouse Spinal Cord on a Density Barrier
Author(s) -
John Graham
Publication year - 2002
Publication title -
the scientific world journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.453
H-Index - 93
eISSN - 2356-6140
pISSN - 1537-744X
DOI - 10.1100/tsw.2002.841
Subject(s) - spinal cord , isolation (microbiology) , fraction (chemistry) , biology , neuroscience , microbiology and biotechnology , chemistry , bioinformatics , chromatography
After a combined enzymic and mechanical disruption of the spinal cord tissue, the low-density motoneurons band at the interface of a 1.06-g/ml barrier through which other contaminating cells sediment.
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