Purification of Peroxisomes Using a Density Barrier in a Swinging-Bucket Rotor | Zendy

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Purification of Peroxisomes Using a Density Barrier in a Swinging-Bucket Rotor

Author(s) -

John Graham

Publication year - 2002

Publication title -

the scientific world journal

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.453

H-Index - 93

eISSN - 2356-6140

pISSN - 1537-744X

DOI - 10.1100/tsw.2002.837

Subject(s) - peroxisome , percoll , organelle , endoplasmic reticulum , mitochondrion , chemistry , cell fractionation , ultrastructure , rotor (electric) , biophysics , microbiology and biotechnology , biochemistry , biology , centrifugation , membrane , physics , anatomy , quantum mechanics , gene

In iodixanol, peroxisomes are the densest organelle in the light mitochondrial fraction and are therefore easily separated from the other components (lysosomes, mitochondria, etc.) in a preformed isosmotic continuous gradient. Because of the large difference in density between peroxisomes and the next densest organelle (mitochondria), a density barrier is effective. The resolution of the peroxisomes is far superior than that in sucrose and, unlike in Percoll, there is no contamination from endoplasmic reticulum.

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