Isolation of Human Platelets (Thrombocytes)
Author(s) -
John Graham
Publication year - 2002
Publication title -
the scientific world journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.453
H-Index - 93
eISSN - 2356-6140
pISSN - 1537-744X
DOI - 10.1100/tsw.2002.820
Subject(s) - platelet , centrifugation , iodixanol , human blood , sedimentation , isolation (microbiology) , differential centrifugation , pellet , whole blood , chemistry , chromatography , biology , immunology , medicine , biochemistry , microbiology and biotechnology , physiology , paleontology , zoology , radiology , sediment , contrast medium
Platelets from human blood can be isolated in high yield by centrifugation of whole blood over an iodixanol density barrier of 1.063 g/ml. The separation from all of the blood cells (which form a pellet) is based on the slower sedimentation velocity of the smaller platelets.
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