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. Cytoplasmic dynein is a principle motor for minus-end directed transport along microtubules[1]. It functions to position organelles and other materials within the cell, and plays an essential role in the separation of chromosomes. Cytoplasmic dynein is a large (1.2 MDa) complex made up of two heavy chain subunits linked by stalk-like domains to a common base. The heavy chains contain the ATP hydrolysis and microtubule binding sites required for motility. Located at the base are a number of subunits thought to regulate the assembly of the complex and its attachment to appropriate cargo. The intermediate chain IC74 forms a key intermediary in the complex, as it is known to associate with the heavy chain and the essential accessory complex dynactin as well as other subunits in the base. We have found that two light chains, LC8 and Tctex-1, attach to the N-terminal domain of IC74 (N-IC74). Here, the interactions between IC74 and the light chains have been characterized by limited proteolysis and mass spectrometry and by NMR spectroscopy. METHODS. The interactions of LC8, Tctex-1 and the N-IC74 from Drosophila melanogaster were characterized in vitro by affinity methods, limited proteolysis, and circular dichroism spectroscopy. Limited digestion of N-IC74 in the presence and absence of LC8, Tctex-1 or both localized binding of the light and intermediate chains to specific segments in the protein. These interactions were further investigated by heteronuclear NMR. RESULTS. Limited tryptic digestion and mass spectrometry[2] localized binding of Tctex-1 to the vicinity of K104 and K105, and localized binding of LC8 to the region downstream of K130. Circular dichroism, fluorescence, sedimentation velocity and proteolysis studies indicate that NIC74 has limited secondary and tertiary structure at near physiological solution conditions. Upon addition of LC8, N-IC74 becomes more ordered. This conformational change is reflected in increased global protection of N-IC74 from proteolytic digestion following the interaction, and in a significant change in the CD signal consistent with a shift from random coil to a more helical conformation. The sequence of IC74 that is responsible for the increase in structure upon binding was identified by heteronuclear NMR using IC74 constructs that contain the binding site, and regions of the protein predicted to fold into a coiled coil structure.

Protein Interactions Within the Cytoplasmic Dynein Complex as Probed by Mass Spectrometry and Nmr