Immobilized Native Bacteria as a Tool for Bioremediation of Soils and Waters: Implementation and Modeling | Zendy

M.C. Lobo | Zendy; María C. Sánchez | Zendy; C. Garbi | Zendy; Esther Ferrer | Zendy; M. J. Martínez-Íñigo | Zendy; J.L. Allende | Zendy; Carmen Enid Martı́nez | Zendy; L. Casasas | Zendy; Ramón AlonsoSanz | Zendy; Alicia Gibello | Zendy; M. Martín | Zendy

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Immobilized Native Bacteria as a Tool for Bioremediation of Soils and Waters: Implementation and Modeling

Author(s) -

M.C. Lobo,

María C. Sánchez,

C. Garbi,

Esther Ferrer,

M. J. Martínez-Íñigo,

J.L. Allende,

Carmen Enid Martı́nez,

L. Casasas,

Ramón AlonsoSanz,

Alicia Gibello,

M. Martín

Publication year - 2002

Publication title -

the scientific world journal

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.453

H-Index - 93

eISSN - 2356-6140

pISSN - 1537-744X

DOI - 10.1100/tsw.2002.211

Subject(s) - bioremediation , bacteria , oligonucleotide , soil water , strain (injury) , chemistry , environmental chemistry , dioxygenase , dna , biology , microbiology and biotechnology , biochemistry , gene , ecology , genetics , anatomy

Based on 3,4-dihydroxyphenylacetate (3,4-DHPA) dioxygenase amino acid sequence and DNA sequence data for homologous genes, two different oligonucleotides were designed. These were assayed to detect 3,4-DHPA related aromatic compound-degrading bacteria in soil samples by using the FISH method. Also, amplification by PCR using a set of ERIC primers was assayed for the detection of Pseudomonas GCH1 strain, which used in the soil bioremediation process. A model was developed to understand and predict the behavior of bacteria and pollutants in a bioremediation system, taking into account fluid dynamics, molecular/cellular scale processes, and biofilm formation.

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