Identification and Characterization of a Minimal Apoptotic Domain from p53 | Zendy

Kevin M. Ryan | Zendy; Karen H. Vousden | Zendy

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Identification and Characterization of a Minimal Apoptotic Domain from p53

Author(s) -

Kevin M. Ryan,

Karen H. Vousden

Publication year - 2001

Publication title -

the scientific world journal

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.453

H-Index - 93

eISSN - 2356-6140

pISSN - 1537-744X

DOI - 10.1100/tsw.2001.174

Subject(s) - identification (biology) , apoptosis , domain (mathematical analysis) , computer science , characterization (materials science) , computational biology , p53 protein , microbiology and biotechnology , chemistry , biology , biochemistry , ecology , nanotechnology , materials science , mathematics , mathematical analysis

METHODS. To further investigate the transcriptionally independent apoptotic functions of p53, we decided to search for a minimal apoptotic domain within the protein. To obtain equal expression of different portions of the p53 protein, we fused all the mutants generated to GFP. These fusion proteins were then expressed in cells and assessed for there ability to induce gene expression by immunoblotting and reporter gene assays, and for their ability to induce programmed cell death by flow cytometry.

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