Open AccessExpression of the Type VI Secretion System 1 Component Hcp1 Is Indirectly Repressed by OpaR inVibrio parahaemolyticus
Author(s) -
Lizhi Ma,
Yiquan Zhang,
Xiaojuan Yan,
Liping Guo,
Li Wang,
Jingfu Qiu,
Ruifu Yang,
Dongsheng Zhou
Publication year - 2012
Publication title -
the scientific world journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.453
H-Index - 93
eISSN - 2356-6140
pISSN - 1537-744X
DOI - 10.1100/2012/982140
Subject(s) - vibrio parahaemolyticus , type vi secretion system , virulence , type three secretion system , biology , transcription (linguistics) , quorum sensing , secretion , regulator , microbiology and biotechnology , transcription factor , gene , vibrio , genetics , bacteria , biochemistry , linguistics , philosophy
The type VI secretion system (T6SS) is bacterial protein injection machinery with roles in virulence, symbiosis, interbacterial interaction, antipathogenesis, and environmental stress responses. There are two T6SS loci, T6SS1 and T6SS2, in the two chromosomes of Vibrio parahaemolyticus , respectively. This work disclosed that the master quorum sensing (QS) regulator OpaR repressed the transcription of hcp1 encoding the structural component Hcp1 of T6SS1 in V. parahaemolyticus , indicating that QS had a negative regulatory action on T6SS1. A single σ 54 -dependent promoter was transcribed for hcp1 in V. parahaemolyticus , and its activity was repressed by the OpaR regulator. Since the OpaR protein could not bind to the upstream region of hcp1 , OpaR would repress the transcription of hcp1 in an indirect manner.
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