Effect of Human and Bovine Serum Albumin on kinetic Chemiluminescence of Mn (III)-Tetrakis (4-Sulfonatophenyl) Porphyrin-Luminol-Hydrogen Peroxide System | Zendy

Sayed Yahya Kazemi | Zendy; Seyed Mohammad Abedirad | Zendy

Open Access

Effect of Human and Bovine Serum Albumin on kinetic Chemiluminescence of Mn (III)-Tetrakis (4-Sulfonatophenyl) Porphyrin-Luminol-Hydrogen Peroxide System

Author(s) -

Sayed Yahya Kazemi,

Seyed Mohammad Abedirad

Publication year - 2012

Publication title -

the scientific world journal

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.453

H-Index - 93

eISSN - 2356-6140

pISSN - 1537-744X

DOI - 10.1100/2012/913412

Subject(s) - chemiluminescence , hydrogen peroxide , luminol , chemistry , porphyrin , bovine serum albumin , human serum albumin , chromatography , albumin , serum albumin , photochemistry , nuclear chemistry , biochemistry

The present work deals with an attempt to study the effect of human and bovine serum albumin on kinetic parameters of chemiluminescence of luminol-hydrogen peroxide system catalyzed by manganese tetrasulfonatophenyl porphyrin (MnTSPP). The investigated parameters involved pseudo-first-order rise and fall rate constant for the chemiluminescence burst, maximum level intensity, time to reach maximum intensity, total light yield, and values of the intensity at maximum CL which were evaluated by nonlinear least square program KINFIT. Because of interaction of metalloporphyrin with proteins, the CL parameters are drastically affected. The systems resulted in Stern-Volmer plots with k Q values of 3.17 × 10 5 and 3.7 × 10 5 M −1 in the quencher concentration range of 1.5 × 10 −6 to 1.5 × 10 −5 M for human serum albumin (HSA) and bovine serum albumin (BSA), respectively.

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