Immobilization of Peroxidase onto Magnetite Modified Polyaniline | Zendy

Eduardo Fernandes Barbosa | Zendy; Fernando Javier Molina | Zendy; Flávio Marques Lopes | Zendy; Pedro Antonio Garcı́a-Ruiz | Zendy; Samantha Salomão Caramori | Zendy; Kátia Flávia Fernandes | Zendy

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Immobilization of Peroxidase onto Magnetite Modified Polyaniline

Author(s) -

Eduardo Fernandes Barbosa,

Fernando Javier Molina,

Flávio Marques Lopes,

Pedro Antonio Garcı́a-Ruiz,

Samantha Salomão Caramori,

Kátia Flávia Fernandes

Publication year - 2012

Publication title -

the scientific world journal

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.453

H-Index - 93

eISSN - 2356-6140

pISSN - 1537-744X

DOI - 10.1100/2012/716374

Subject(s) - horseradish peroxidase , polyaniline , glutaraldehyde , magnetite , peroxidase , chemistry , nuclear chemistry , chemical engineering , chromatography , materials science , biochemistry , enzyme , polymer , organic chemistry , engineering , metallurgy , polymerization

The present study describes the immobilization of horseradish peroxidase (HRP) on magnetite-modified polyaniline (PANImG) activated with glutaraldehyde. After the optimization of the methodology, the immobilization of HRP on PANImG produced the same yield (25%) obtained for PANIG with an efficiency of 100% (active protein). The optimum pH for immobilization was displaced by the effect of the partition of protons produced in the microenvironment by the magnetite. The tests of repeated use have shown that PANImG-HRP can be used for 13 cycles with maintenance of 50% of the initial activity.

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