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Background . The aim of this paper was to determine the influence of different  in vitro  culture media on mRNA expression of Hedgehog genes,  il-6,  and important genes regarding reactive oxygen species in single mouse embryos.  Methods . Reverse transcription of single embryos either cultured  in vitro  from day 0.5 until 3.5 (COOK's Cleavage medium or Vitrolife's G-1 PLUS medium) or  in vivo  until day 3.5  post coitum . PCR was carried out for   β  - actin  followed by nested-PCR for  shh, ihh, il-6, nox, gpx4, gpx1,  and  prdx2 .  Results . The number of murine blastocysts cultured in COOK medium which expressed  il-6, gpx4, gpx1,  and  prdx2  mRNA differed significantly compared to the  in vivo  group. Except for  nox , the mRNA profile of the Vitrolife media group embryos varied significantly from the  in vivo  ones regarding the number of blastocysts expressing the mRNA of  shh, ihh, il-6, gpx4, gpx1  and  prdx2 .  Conclusions . The present study shows that different  in vitro  culture media lead to different mRNA expression profiles during early development. Even the newly developed  in vitro  culture media are not able to mimic the female reproductive tract. The question of long-term consequences for children due to assisted reproduction techniques needs to be addressed in larger studies.

the scientific world journal/thescientificworldjournal

Selection of the<i>In Vitro</i>Culture Media Influences mRNA Expression of Hedgehog Genes,<i>Il-6,</i>and Important Genes regarding Reactive Oxygen Species in Single Murine Preimplantation Embryos

Selection of theIn VitroCulture Media Influences mRNA Expression of Hedgehog Genes,Il-6,and Important Genes regarding Reactive Oxygen Species in Single Murine Preimplantation Embryos