Open AccessVesiculation fromPseudomonas aeruginosaunder SOS
Author(s) -
Reshma Maredia,
Navya Devineni,
Peter S. Lentz,
Shatha F. Dallo,
JiehJuen Yu,
Neal Guentzel,
James P. Chambers,
Bernard P. Arulanandam,
William E. Haskins,
Tao Weitao
Publication year - 2012
Publication title -
the scientific world journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.453
H-Index - 93
eISSN - 2356-6140
pISSN - 1537-744X
DOI - 10.1100/2012/402919
Subject(s) - repressor lexa , pseudomonas aeruginosa , microbiology and biotechnology , cytotoxicity , sos response , ciprofloxacin , bacterial outer membrane , antibiotics , chemistry , bacteria , biology , in vitro , escherichia coli , biochemistry , gene , genetics , repressor , transcription factor
Bacterial infections can be aggravated by antibiotic treatment that induces SOS response and vesiculation. This leads to a hypothesis concerning association of SOS with vesiculation. To test it, we conducted multiple analyses of outer membrane vesicles (OMVs) produced from the Pseudomonas aeruginosa wild type in which SOS is induced by ciprofloxacin and from the LexA noncleavable ( lexAN ) strain in which SOS is repressed. The levels of OMV proteins, lipids, and cytotoxicity increased for both the treated strains, demonstrating vesiculation stimulation by the antibiotic treatment. However, the further increase was suppressed in the lexAN strains, suggesting the SOS involvement. Obviously, the stimulated vesiculation is attributed by both SOS-related and unrelated factors. OMV subproteomic analysis was performed to examine these factors, which reflected the OMV-mediated cytotoxicity and the physiology of the vesiculating cells under treatment and SOS. Thus, SOS plays a role in the vesiculation stimulation that contributes to cytotoxicity.
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