Assessment of Tools for Marker-Assisted Selection in a Marine Commercial Species: Significant Association between MSTN-1 Gene Polymorphism and Growth Traits | Zendy

I. Sánchez-Ramos | Zendy; Ismael Cross | Zendy; J Mácha | Zendy; Gonzalo MartínezRodríguez | Zendy; Vladimír Krylov | Zendy; Laureana Rebordinos | Zendy

Open Access

Assessment of Tools for Marker-Assisted Selection in a Marine Commercial Species: Significant Association between MSTN-1 Gene Polymorphism and Growth Traits

Author(s) -

I. Sánchez-Ramos,

Ismael Cross,

J Mácha,

Gonzalo MartínezRodríguez,

Vladimír Krylov,

Laureana Rebordinos

Publication year - 2012

Publication title -

the scientific world journal

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.453

H-Index - 93

eISSN - 2356-6140

pISSN - 1537-744X

DOI - 10.1100/2012/369802

Subject(s) - biology , genetics , quantitative trait locus , candidate gene , marker assisted selection , gene , genetic marker , linkage disequilibrium , genotype , restriction fragment length polymorphism , polymorphism (computer science) , allele , haplotype

Growth is a priority trait from the point of view of genetic improvement. Molecular markers linked to quantitative trait loci (QTL) have been regarded as useful for marker-assisted selection in complex traits as growth. Polymorphisms have been studied in five candidate genes influencing growth in gilthead seabream ( Sparus aurata ): the growth hormone ( GH ), insulin-like growth factor-1 ( IGF-1 ), myostatin ( MSTN-1 ), prolactin ( PRL ), and somatolactin ( SL ) genes. Specimens evaluated were from a commercial broodstock comprising 131 breeders (from which 36 males and 44 females contributed to the progeny). In all samples eleven gene fragments, covering more than 13,000 bp, generated by PCR-RFLP, were analyzed; tests were made for significant associations between these markers and growth traits. ANOVA results showed a significant association between MSTN-1 gene polymorphism and growth traits. Pairwise tests revealed several RFLPs in the MSTN-1 gene with significant heterogeneity of genotypes among size groups. PRL and MSTN-1 genes presented linkage disequilibrium. The MSTN-1 gene was mapped in the centromeric region of a medium-size acrocentric chromosome pair.

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