Identification of the thymidylate synthase within the genome of white spot syndrome virus | Zendy

Qin Li | Zendy; Pan Deng | Zendy; Jinghai Zhang | Zendy; Feng Yang | Zendy

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Identification of the thymidylate synthase within the genome of white spot syndrome virus

Author(s) -

Qin Li,

Pan Deng,

Jinghai Zhang,

Feng Yang

Publication year - 2004

Publication title -

journal of general virology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.55

H-Index - 167

eISSN - 1465-2099

pISSN - 0022-1317

DOI - 10.1099/vir.0.80048-0

Subject(s) - biology , white spot syndrome , thymidylate synthase , escherichia coli , gene , genome , open reading frame , recombinant dna , phylogenetic tree , virology , microbiology and biotechnology , homology (biology) , virus , southern blot , peptide sequence , genetics , fluorouracil , chemotherapy

Thymidylate synthase (TS) (EC 2.1.1.45) is essential for the de novo synthesis of dTMP in prokaryotic and eukaryotic organisms. Within the white spot syndrome virus (WSSV) genome, an open reading frame (WSV067) that encodes a 289 amino acid polypeptide showed significant homology to all known TSs from species including mammals, plants, fungi, protozoa, bacteria and DNA viruses. In this study, WSV067 was expressed in Escherichia coli, and the purified recombinant protein showed TS activity in dUMP-folate-binding assays using ultraviolet difference spectroscopy. RT-PCR and Western blot analyses showed that WSV067 was a genuine and early gene. Phylogenetic analysis revealed that WSSV-TS was more closely related to the TSs of eukaryotes than to those from prokaryotes.

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