Open AccessCharacterization of formaldehyde-inactivated poliovirus preparations made from live-attenuated strains
Author(s) -
Javier Martín,
Graham Crossland,
David J. Wood,
Philip D. Minor
Publication year - 2003
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/vir.0.19088-0
Subject(s) - poliovirus , immunogenicity , virology , biology , infectivity , context (archaeology) , microbiology and biotechnology , inactivated poliovirus vaccine , virus , poliomyelitis , attenuated vaccine , immune system , virulence , immunology , gene , biochemistry , paleontology
Formaldehyde-inactivated virus samples from type 1 poliovirus live-attenuated strains were prepared in the laboratory. The effect of treatment with formaldehyde on virus infectivity and immunogenicity in mice was investigated and the results compared with those from Mahoney wild-type poliovirus strain, the common type 1 component in commercial inactivated polio vaccines (IPV). Differences in the potency and specificity between these experimental vaccines were identified in both normal mice and transgenic mice expressing the human poliovirus receptor. The possible advantages/disadvantages of using live-attenuated strains for IPV production are discussed in the context of the global polio eradication initiative. A novel transgenic mouse model to study in vivo the immune protection induced by IPV preparations is described.
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