Open AccessInfection competition against grouper nervous necrosis virus by virus-like particles produced in Escherichia coli
Author(s) -
Ming–Wei Lu,
Wangta Liu,
Chan-Shing Lin
Publication year - 2003
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/vir.0.18649-0
Subject(s) - biology , capsid , virus , grouper , virology , virus like particle , escherichia coli , recombinant dna , microbiology and biotechnology , gene , genetics , fish <actinopterygii> , fishery
Dragon grouper (Epinephelus lanceolatus) nervous necrosis virus (DGNNV) comprises 180 copies of capsid protein that encapsulate a bipartite genome of single-stranded (+)-RNAs. This study reports that virus-like particles (VLPs) are formed in Escherichia coli expressing the full-length ORF encoding the DGNNV capsid protein. Two sizes of VLPs are observed. The heavier particles resemble the native piscine nodavirus in size and stain permeability, while the lighter ones are approximately two-thirds of the full size. The recombinant VLPs block attachment of native virus to the surface of cultured fish nerve cells, blocking infection by the native virus.
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